The generation of magnonic spin current by the spin Seebeck effect (SSE) and that of spin-polarized current by the anomalous Nernst effect (ANE) are the most fascinating phenomena in the field of spin caloritronics. There are several methods to generate the temperature gradient (∇T), including Peltier heating, external heater, microwave heating, and light heating. Among them, light energy is one of the richest thermal energy harvesting sources. In this work, we systematically investigate the thermal spin current (JS) driven by light in the framework of the SSE and the ANE, respectively. Unlike JS induced by electric heating methods following ∇T, the JS can be enhanced, reduced, and even reversed via the SSE under certain light frequency and the thickness of magnetic layers. Most importantly, by flipping the direction of the incident light, we are able to qualitatively distinguish the interface and bulk contributions to the transverse spin accumulation for the first time. Interestingly, we find that the derived interfacial and bulk spin Seebeck coefficient is frequency independent. Thus, unlike the conventional electrical heating, light offers distinct heating mechanism to develop spintronic and spin caloritronic devices.